Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (Drosophila melanogaster) |
VT064565-x-VT043699 | this paper | splitgal4.janelia.org: SS36564 | split-GAL4 driver line targeting aIPg (aIPgSS1) |
| Genetic reagent (Drosophila melanogaster) |
VT043699-x-R11A07 | this paper | splitgal4.janelia.org: SS36551 | split-GAL4 driver line targeting aIPg (aIPgSS2) |
| Genetic reagent (Drosophila melanogaster) |
R11A07-x-VT043700 | this paper | splitgal4.janelia.org: SS32237 | split-GAL4 driver line targeting aIPg (aIPgSS3) |
| Genetic reagent (Drosophila melanogaster) |
R72C11-x-R11A07 | this paper | splitgal4.janelia.org: SS47478 | split-GAL4 driver line targeting aIPg (aIPgSS4) |
| Genetic reagent (Drosophila melanogaster) |
R11A07-x-R72C11 | this paper | splitgal4.janelia.org: SS56964 | split-GAL4 driver line targeting aIPg (aIPgSS5) |
| Genetic reagent (Drosophila melanogaster) |
R35C10-x-R71A09 | this paper | splitgal4.janelia.org: SS56987 | split-GAL4 driver line targeting pC1d (pC1dSS1) |
| Genetic reagent (Drosophila melanogaster) |
R71A09-x-R26F09 | this paper | splitgal4.janelia.org: SS57598 | split-GAL4 driver line targeting pC1d (pC1dSS2) |
| Genetic reagent (Drosophila melanogaster) |
VT25602-x-VT002064 | this paper | splitgal4.janelia.org: SS43274 | split-GAL4 driver line targeting pC1d and pC1e (pC1dSS3) |
| Genetic reagent (Drosophila melanogaster) |
R35C10-x-VT002063 | this paper | splitgal4.janelia.org: SS59331 | split-GAL4 driver line targeting pC1d and pC1e (pC1dSS4) |
| Genetic reagent (Drosophila melanogaster) |
R35C10-x-R60G08 | this paper | splitgal4.janelia.org: SS59336 | split-GAL4 driver line targeting pC1e (pC1eSS1) |
| Genetic reagent (Drosophila melanogaster) |
R60G08-x-VT002063 | this paper | splitgal4.janelia.org: SS39313 | split-GAL4 driver line targeting pC1e (pC1eSS2) |
| Genetic reagent (Drosophila melanogaster) |
R60G08-x-R35C10 | this paper | splitgal4.janelia.org: SS59433 | split-GAL4 driver line targeting pC1e (pC1eSS3) |
| Genetic reagent (Drosophila melanogaster) |
R18A05-x-dsx-ZpGal4DBD | this paper; gift of K. Wang and B. Dickson |
SS75230 |
split-GAL4 driver line targeting pC1a-c |
| Genetic reagent (Drosophila melanogaster) |
BPp65AD-x-BPZpGal4DBD |
Pfeiffer et al., 2010; Aso et al., 2014 |
emptySS |
split-GAL4 driver line empty brain control |
| Genetic reagent (Drosophila melanogaster) |
20XUAS-CsChrimson-mVenus |
Klapoetke et al., 2014; Aso et al., 2014 |
||
| Genetic reagent (Drosophila melanogaster) |
10XUAS-CsChrimson-mVenus |
Klapoetke et al., 2014; Aso et al., 2014 |
||
| Genetic reagent (Drosophila melanogaster) |
5XUAS-CsChrimson-mVenus |
Klapoetke et al., 2014; Aso et al., 2014 |
||
| Genetic reagent (Drosophila melanogaster) |
MCFO-1 | Nern et al., 2015 | ||
| Genetic reagent (Drosophila melanogaster) |
pJFRC2-10XUAS-IVS-mCD8::GFP | Pfeiffer et al., 2010 | ||
| Genetic reagent (Drosophila melanogaster) |
UAS-impTNT-HA | Sweeney et al., 1995 | ||
| Genetic reagent (Drosophila melanogaster) |
UAS-TNT-E | Sweeney et al., 1995 | ||
| Antibody | nc82 supernatant; Mouse α-bruchpilot monoclonal |
Developmental Studies Hybridoma Bank |
Cat #: nc82-s |
https://www.janelia.org/project-team/flylight/protocols
Dilution, (1:30) |
| Antibody | Mouse α-V5 Tag monoclonal | AbD Serotec | Cat #: MCA1360D550GA | Dilution (1:500) |
| Antibody | Rabbit α-HA Tag monoclonal | Cell Signaling Technologies |
Cat #: 3724S | Dilution (1:300) |
| Antibody | Rat α-FLAG Tag (DYKDDDDK Epitope tag) monoclonal |
Novus Biologicals | Cat #: NBP1-06712 | Dilution (1:200) |
| Antibody | rabbit anti-GFP polyclonal | Thermo Fisher Scientific | Cat #: A-11122; RRID:AB_221569 |
Dilution, (1:1000) |
| Antibody | Alexa Fluor 488 goat anti-rabbit polyclonal |
Thermo Fisher Scientific | Cat #: A-11034; RRID:AB_2576217 |
Dilution, (1:800) |
| Antibody | Alexa Fluor 568 goat anti-mouse polyclonal |
Thermo Fisher Scientific | Cat #: A-11031; RRID:AB_144696 |
Dilution, (1:400) |
| Antibody | ATTO 647N goat anti-rat polycolonal |
Rockland Immunochemicals Inc |
Cat #: AB_10893386 | Dilution, (1:300) |
| Chemical compound, drug |
paraformaldehyde | Electron Microscopy Sciences |
15713 s | https://www.janelia.org/project-team/flylight/protocols |
| Chemical compound, drug |
Triton X-100 | Sigma–Aldrich | X100 | https://www.janelia.org/project-team/flylight/protocols |
| Chemical compound, drug |
DPX Mountant | Electron Microscopy Sciences |
#13512 | https://www.janelia.org/project-team/flylight/protocols |
| Chemical compound, drug |
xylene | Fisher Scientific | x5-500 | https://www.janelia.org/project-team/flylight/protocols |