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. 2020 Dec 22;16(12):e1009126. doi: 10.1371/journal.ppat.1009126

Fig 1. PMZ does not affect bacterial growth nor viability.

Fig 1

100 μM promethazine (PMZ) slightly influenced the structure, but not the viability of biofilm-grown P. aeruginosa cells. (a) Representative maximum intensity projections of PA14 biofilms treated with PMZ for 24 h in comparison to untreated control biofilms. The biofilms were stained with the BacLight Bacterial Viability Kit, visualizing dead cells in red (propidium iodide) and living cells in green (Syto9). (b) Determination of colony forming units (CFU) from the untreated (dark grey) and PMZ-treated (light grey) biofilms revealed no significant differences in CFU/viability (Significance was calculated using the t- test). Data show mean CFU counts from overall 6 independent experiments. (c) Increasing concentrations of PMZ (1 μM, 10 μM, 25 μM and 100 μM) marginally affected planktonic growth of P. aeruginosa PA14 cells. Experiments were performed three times with at least three biological replicates each; here an exemplary data set from one experiment is shown.