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. 2020 Dec 22;16(12):e1009126. doi: 10.1371/journal.ppat.1009126

Fig 6. High extracellular potassium and the addition of CCCP affect the synergistic anti-biofilm activity of PMZ in combination with tobramycin.

Fig 6

P. aeruginosa PA14 biofilms were treated with increasing concentrations of tobramycin (TOB) alone or in combination with 100 μM PMZ and further challenged with the protonophore CCCP (a), or with high extracellular potassium (300 mM KCl) (b). Colony forming units (CFU) were determined from the tobramycin treated (dark grey) and tobramycin plus PMZ treated (light grey) biofilms. Isopropanol treatment (IPA) was used as a killing control. Mean CFU counts were performed on pools of three wells in at least three independent experiments. Error bars represent the standard error of the mean, while the dashed line indicates the lower limit of detection of the assay.