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. 2020 Dec 7;2020:6697577. doi: 10.1155/2020/6697577

Figure 3.

Figure 3

Under stress conditions, USCs-exos suppressed the expression of GRP78 and GRP94. Except the control group, NP cells were cultured for 48 h under pressure. USCs-exos-10, 50, and 100 indicated that 10, 50, or 100 μg/ml exosomes were added to each response group. (a) Protein levels of GRP78 and GRP94 were measured by Western blot analysis, and their relative quantities were calculated (b, c) using β-actin as an internal reference. (d) Western blot analysis was used to detect the expression levels of caspase-3 and caspase-12, and their relative quantities (e, f) were calculated using β-actin as an internal reference. (g) Fluorescence images of TUNEL analysis in different groups. The nucleus was stained with DAPI. (h) The proportion of apoptotic cells according to TUNEL staining. Data are expressed as the mean ± SD. Compared to the control group, p < 0.05; #compared to the 48 h group, p < 0.05.