TEAS on PND7-PND13 did not improve the development and USVs of VPA-induced offspring. (a) Experimental design of TEAS intervention and behavior tests. (b) The photo is the offspring receiving TEAS. (c) The number of pups with eye-opened on certain days (two-way ANOVA, NS-control n = 30, VPA-sham n = 24, VPA-TEAS n = 21). (d) Body weight of pups from PND2 to PND21 (one-way ANOVA, NS-control n = 30, VPA-sham n = 24, VPA-TEAS n = 21). (e) Total USV numbers (PND7 vs. PND13: paired t-test, NS-control vs. VPA-sham vs. VPA-TEAS: one-way ANOVA, NS-control n = 15, VPA-sham n = 14, VPA-TEAS n = 11). Data are presented as the mean ± SEM. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001; NS-control vs. VPA-TEAS, #P < 0.05, ##P < 0.01.