Table 1.
Haematology and coagulation parameters in rats following 90 days of treatment with Phytase TSP concentrate.
| Parameter (units) | Sex | Control 0 mg/kg bw/d |
Low-dose 100 mg/kg bw/d |
Mid-dose 500 mg/kg bw/d |
High-dose 1000 mg/kg bw/d |
|---|---|---|---|---|---|
| Haematology | |||||
| WBC (109/l) | M | 10.87 ± 1.99 | 10.87 ± 2.40 | 11.72 ± 4.21 | 10.96 ± 2.33 |
| F | 5.67 ± 1.10 | 6.16 ± 1.85 | 5.78 ± 1.68 | 6.15 ± 1.71 | |
| LYM (109/l) | M | 6.515 ± 1.576 | 5.670 ± 1.796 | 6.055 ± 1.719 | 6.374 ± 1.708 |
| F | 3.613 ± 0.766 | 3.837 ± 1.220 | 3.604 ± 1.149 | 4.035 ± 1.393 | |
| MON (109/l) | M | 0.399 ± 0.093 | 0.582 ± 0.235 | 0.609 ± 0.344* | 0.439 ± 0.099 |
| F | 0.157 ± 0.051 | 0.186 ± 0.076 | 0.184 ± 0.059 | 0.152 ± 0.044 | |
| NEU (109/l) | M | 3.495 ± 0.498 | 4.162 ± 0.570* | 3.743 ± 0.766 | 3.681 ± 0.667 |
| F | 1.694 ± 0.373 | 1.885 ± 0.572 | 1.725 ± 0.524 | 1.713 ± 0.360 | |
| EOS (109/l) | M | 0.275 ± 0.068 | 0.314 ± 0.076 | 0.280 ± 0.109 | 0.293 ± 0.121 |
| F | 0.138 ± 0.052 | 0.180 ± 0.068 | 0.187 ± 0.089 | 0.179 ± 0.048 | |
| BAS (109/l) | M | 0.175 ± 0.051 | 0.184 ± 0.086 | 0.230 ± 0.171 | 0.167 ± 0.068 |
| F | 0.055 ± 0.022 | 0.065 ± 0.032 | 0.062 ± 0.038 | 0.067 ± 0.033 | |
| RBC (1012/l) | M | 9.149 ± 0.306 | 9.267 ± 0.382 | 8.784 ± 0.878 | 8.899 ± 0.512 |
| F | 8.130 ± 0.272 | 8.409 ± 0.437 | 8.275 ± 0.424 | 8.157 ± 0.265 | |
| HGB (g/l) | M | 169.7 ± 4.5 | 171.3 ± 6.3 | 162.2 ± 20.3 | 165.8 ± 9.1 |
| F | 158.5 ± 5.8 | 159.8 ± 11.1 | 160.1 ± 7.5 | 158.6 ± 5.2 | |
| HCT (l/l) | M | 0.4811 ± 0.0146 | 0.4857 ± 0.0142 | 0.4612 ± 0.0565 | 0.4733 ± 0.0275 |
| F | 0.4418 ± 0.0169 | 0.4491 ± 0.0303 | 0.4475 ± 0.0222 | 0.4447 ± 0.0158 | |
| MCV (fl) | M | 52.6 ± 1.1 | 52.4 ± 2.0 | 52.2 ± 2.0 | 53.1 ± 1.3 |
| F | 54.3 ± 1.2 | 53.4 ± 1.3 | 54.2 ± 1.3 | 54.5 ± 1.4 | |
| MCH (pg) | M | 18.56 ± 0.33 | 18.50 ± 0.81 | 18.44 ± 0.78 | 18.65 ± 0.56 |
| F | 19.51 ± 0.48 | 18.96 ± 0.54* | 19.38 ± 0.47 | 19.46 ± 0.53 | |
| MCHC (g/l) | M | 353.0 ± 2.5 | 352.5 ± 3.0 | 352.0 ± 3.1 | 350.4 ± 2.9* |
| F | 358.9 ± 4.2 | 355.8 ± 2.3 | 358.2 ± 2.2 | 356.7 ± 4.0 | |
| PLT (109/l) | M | 806.9 ± 85.9 | 820.1 ± 82.2 | 862.8 ± 135.4 | 751.9 ± 70.3 |
| F | 800.1 ± 93.6 | 821.8 ± 78.3 | 789.8 ± 122.0 | 765.1 ± 108.9 | |
| Coagulation | |||||
| PT (sec) | M | 14.76 ± 1.32 | 15.51 ± 1.51 | 15.73 ± 1.59 | 14.15 ± 1.64 |
| F | 15.09 ± 0.67 | 13.26 ± 0.64* | 14.80 ± 1.20 | 14.19 ± 1.05* | |
| APTT (sec) | M | 12.56 ± 3.19 | 13.35 ± 2.30 | 14.24 ± 5.60 | 13.34 ± 2.30 |
| F | 12.21 ± 1.96 | 11.51 ± 1.82 | 11.60 ± 1.44 | 11.75 ± 2.62 | |
WBC, white blood cell count; LYM, lymphocyte count; MON, monocyte count; NEU, neutrophil count; EOS, eosinophil count; BAS, basophil count; RBC, red blood cell count; HGB, haemoglobin; HCT, Haematocrit; MCV, mean corpuscular volume; MCH, mean corpuscular haemoglobin; MCMC, mean corpuscular haemoglobin concentration; PLT, platelet count; PT, prothrombin time; APTT, activated partial thromboplastin time. Values are mean ± SD (n = 15 per group in the control and high-dose groups, n = 10 in the low- and mid-dose groups.
*
Indicates statistically significant difference from control diet p<0.05.