Inhibition of miR-199a attenuated hypertrophy of neonatal rat ventricular cardiomyocytes
(A) The expression of miR-199a in NRVMs with or without miR-199a inhibitor treatment (n = 3). (B) Representative confocal images of sarcomeric alpha actinin (SAA, red) and DAPI (nuclei, blue) staining of NRVMs in different groups. NRVM size in each group was calculated by ImageJ. Scale bar, 20 μm. Approximately 200 counted cells in each group. (C) The expression of hypertrophic markers ANP, BNP, β-MHC, and Acta1 in each NRVM group (n = 3). ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.