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. 2020 Nov 17;23:406–417. doi: 10.1016/j.omtn.2020.11.007

Figure 3.

Figure 3

AAV9-anti-miR-199a TuDs virus attenuated cardiac hypertrophy

(A) The representative gross morphology (scale bars, 1 mm) and H&E staining (scale bars, 400 μm) of heart sections in control and cardiac hypertrophy mice injected with AAV9-anti-miR-199a TuDs or AAV9-EGFP. n = 8. (B) The ratio of heart weight (HW, mg) and tibia length (TL, mm) in different mouse groups. n = 8. (C) Representative images of WGA (green) and DAPI (nuclei, blue) staining of hearts in different mouse groups (left). Scale bars, 20 μm. Calculated relative cell areas of cardiomyocytes in different mouse groups (right). n = 8. (D) Representative M-mode echocardiography images in different mouse groups (top). The percentages of left ventricular ejection fraction (LVEF) and left ventricular fractional shortening (LVFS) in the different mouse groups (bottom). n = 8. (E) The expression of hypertrophic markers ANP, BNP, β-MHC, and Acta1 in different mouse groups. n = 8. (F) The expression of miR-199a in different mouse groups. n = 8 in each group. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.