Figure 5.

Validation of PGC-1α as a target of miR-199a in cardiac hypertrophy

(A) The expression of six predicted targets of miR-199a in hypertrophic NRVMs with and without miR-199a inhibitor treatment (n = 3). (B) The expression of six predicted targets of miR-199a in cardiac hypertrophy mice and the anti-miR-199a-treated mice (n = 8). (C) Schematic representation of genomic localization of miR-199a binding site in the 3′UTR of Ppargc1a gene and the conserved seed sequences in different species. (D) Dual luciferase activity assay of H293T cells co-transfected miR-199a mimic or mimic control with Luc-PGC-1α-3′UTR reporter plasmid or Luc-PGC-1α-Mut-3′UTR plasmid (n = 4). ∗p < 0.05, ∗∗p < 0.01.