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. 2020 Sep 13;34(1):14–27. doi: 10.1007/s13577-020-00421-y

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — FOXO3 was the target gene for miR-223-3p. a Sequences of FOXO3-WT (top), miR-223-3p (middle), and FOXO3-MUT (below) were listed. b, c Dual-luciferase reporter assay showed that FOXO3 was the target for miR-223-3p. All experiments have been performed in independent triplicate and experimental data were expressed as mean ± standard deviation (SD). *P < 0.05, ***P < 0.001, vs. Blank. WT wild type, MUT mutated