Fig. 1.

Mutation of OsMS188 to obtain complete male-sterile mutants. a Gene structure and the editing site of OsMS188. The black box indicates the exon. The black arrow indicates the site of the PAM sequence. The red box around the sequence shows the mutation sites in b. b Mutation sites of OsMS188 in the wild type (WT), osms188–1 and osms188–2; the red font indicates the variation in nucleic acids and amino acids. c-e Phenotype of WT plant (c), osms188–1 mutant (d) and osms188–2 mutant (e). f-n Spikelets, mature anthers and Alexander staining of the WT plant (f-h), osms188–1 mutant (i-k) and osms188–2 mutant (l-n). Bars: (c-e) 20 cm; (f, i, l) 1 mm; (g-h, j-k, m-n) 200 μm