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. 2021 Jan 6;14:2. doi: 10.1186/s12284-020-00446-9

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Promoter-GUS analysis and relative expression level of OsAAP4. OsAAP4 promoter-GUS staining in the root tip (a), lateral root (b), adventitious roots (c), short outgrowth bud (d), long outgrowth bud (e), leaf blade (f), leaf sheath (g), stem (h), and panicle (i) using the Hap2-Indica type of pOsAAP4-GUS-transgenic plants. Transverse section of a root (j) and its enlargement (k), leaf sheath (l), leaf blade (m), stem (n), and panicle (o) using the Hap2-Indica type of pOsAAP4-GUS transgenic plants. p The expression pattern of OsAAP4 in different tissues of Japonica ZH11. The primers used for quantifying OsAAP4a expression was F: TGGCACTCACCCTTGCACAC, and R: CCGTCCACACCGTCCCTTGT, for quantifying OsAAP4b expression was ACTTGAGCTCTCTGCATTGGGT, and R: AGCGGTAGCAATTGGCGAGGA, and for quantifying OsAAP4b + c expression was TTGCTGCAGGTGTTCGCGCA, ATCGTCCGCAGCACCAGCTTCAG which primers were designed for common sequences between two splice variants OsAAP4b and OsAAP4c. OsAAP4c of the two splice variants accounts for half of the expression level for both the splice variants. Pe indicates pericycle, V indicates vascular, X indicates xylem, and P indicates phloem in (k-o). Scale bars, 0.5 cm (a-c, f-h), 0.2 cm (d), 0.1 cm (e, i), 50.0 μm (j), 20.0 μm (k-o)