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. 2021 Jan 6;14:2. doi: 10.1186/s12284-020-00446-9

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Phenotypic analysis of OsAAP4 transgenic plants in the Japonica ZH11 background grown in Wuhan paddy fields. Whole-plant phenotype (a), grain yield phenotype (b), relative expression of OsAAP4 in the leaf blade (c), tiller number per plant (d), filled grain number per plant (e), grain yield per plant (f) and nitrogen utilization efficiency (NUtE) of transgenic plants and ZH11 (g). OEa-1 and OEa-2 indicate long variants of OsAAP4a-overexpressing lines, OEb-1 and OEb-2 indicate short variants OsAAP4b-overexpressing lines, and Ri-1 and Ri-2 indicate OsAAP4-RNAi lines. The primers used for quantifying OsAAP4a expression was F: TGGCACTCACCCTTGCACAC, and R: CCGTCCACACCGTCCCTTGT, for quantifying OsAAP4b expression was ACTTGAGCTCTCTGCATTGGGT, and R: AGCGGTAGCAATTGGCGAGGA. The letters above the error bars are ranked by the T test, “**” indicates a significant difference at p < 0.01, and “***” indicates a significant difference at p < 0.001. Scale bar, 10.0 cm (a), 2.0 cm (b). Values are means ± s.d. (n > 20)