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. 2021 Jan 6;14:2. doi: 10.1186/s12284-020-00446-9

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — Protoplast amino acid-uptake assay among ZH11, OEa, OEb, and Ri lines. Fluorescence was detected after culturing protoplasts with FITC-labeled amino acids for 4 h. Green fluorescence images of ZH11 and OEa, OEb and Ri lines under treatment with 0.5 mM Val-FITC (a), 0.5 mM Pro-FITC (b), 2.0 mM Val-FITC (c), and 2.0 mM Pro-FITC (d). e Detection of cell fluorescence signal intensity in (a-d). Fluorescence intensities were normalized to the area of the respective cell by ImageJ software, and a total of 100 cells were statistically analyzed. Scale bars, 5.0 μm (a-d). The letters above the error bars are ranked by the T test, “*” indicates a significant difference at p < 0.05, and “**” indicates a significant difference at p < 0.01. Values are means ± s.d. (n = 3)