3D culture media (for 3D co-culture of type II cells and Mlg)	Final concentration	Amount
DMEM/F-12	–	450 mL
HEPES buffer (1 M)	25 mM	12.5 mL
FBS (heat inactivated)	10%	50 mL
Pen-strep	100 U/mL penicillin, 100 μg/mL streptomycin	5 mL
Antibiotic-Antimycotic	100 U/mL penicillin, 100 μg/mL streptomycin, 0.25 μg/mL amphotericin B	5 mL
Gentamicin and amphotericin	10 μg/mL gentamicin 2.5 μg/mL amphotericin	5 mL
ITS, insulin, transferrin, and selenium	1×	5 mL
Total	n/a	500 mL

Keep sterile and store at 4°C for 4 weeks.