REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
pro-Sp-C (rabbit, 1:500)	Millipore	Cat# AB3786, RRID:AB_91588
T1α (hamster, 1:50)	Developmental Studies Hybridoma Bank	Cat# 8.1.1, RRID:AB_531893
RFP (rabbit, 1:200)	Rockland Immunochemicals	Cat# 600-401-379, RRID:AB_1182807AB_2209751
GFP (chicken, 1:500)	Aves Laboratories	Cat# GFP-1020, RRID:AB_10000240
HopX (mouse, 1:50)	Santa Cruz Biotechnology	Cat# sc-398703, RRID:AB_2687966
Ager (rat, 1:50)	R&D Systems	Cat# MAB1179, RRID:AB_2289349
Biotin-EpCAM (CD326, for cell isolation, rat, 1:50)	Thermo Fisher Scientific	Cat# 13-5791-82, RRID:AB_1659713
CD45 (rat, 42 μg/plate)	BioLegend	Cat# 103101, RRID:AB_312966
CD32 (rat, 16 μg/plate)	BioLegend	Cat# 156402, RRID:AB_2783133
Streptavidin Particles Plus – DM (RUO)	BD Biosciences	Cat# 557812, RRID:AB_10050580
Chemicals, peptides, and recombinant proteins
Tamoxifen	Sigma-Aldrich	Cat# T5648
Dispase	Corning	Cat# 354235
JTE-013	Cayman Chemical	Cat# 10009458
Matrigel	Corning	Cat# 354230
SYBR green	Roche	Cat# 04913914001
Sphingosine-1-phosphate	Cayman Chemical	Cat# 62570
DNase I	Sigma	Cat# D5025
Bovine serum albumin (BSA)	MP Biomedicals	Cat# 9048-46-8
Bovine serum albumin (BSA, fatty acid free)	Sigma	Cat# A8806
Trypan blue	Lonza	Cat# 17-942E
Paraformaldehyde	Sigma	Cat# P6148
Agarose	Dot Scientific	Cat# CGLE-500
DAPI	Sigma	Cat# D9542
Dextran-coated charcoal	Sigma	Cat# C6241
Dulbecco's modified Eagle’s medium (DMEM)	Corning	Cat# 10-013-CV
HEPES	Thermo Fisher Scientific	Cat# 15630106
Fetal bovine serum (FBS, heat inactivated)	Hyclone	Cat# SH30910.03
Penicillin-streptomycin	Thermo Fisher Scientific	Cat# 15140122
Gentamicin	Sigma	Cat# G1397
Amphotericin	Thermo Fisher Scientific	Cat# 15290018
Insulin-transferrin-selenium (ITS)	Sigma	Cat# I1884-1VL
Antibiotic-antimycotic (Anti-Anti)	Thermo Fisher Scientific	Cat# 15240062
Dulbecco's modified Eagle’s medium: nutrient mixture F-12 (DMEM/F-12)	Thermo Fisher Scientific	Cat# 4192001
Corning collagen I, rat	Corning	Cat# 354236
RBC lysis buffer	eBioscience	Cat# 00-4333-57
Eagle's minimum essential medium (EMEM)	ATCC	Cat# 30-2003
Critical commercial assays
RNeasy mini kit	Qiagen	Cat# 74104
Experimental models: cell lines
Mlg mouse lung fibroblast cells	ATCC	CCL-206
Experimental models: organisms/strains
Mouse: SpC-CreER (Sfptctm(cre/ERT2)Blh)	From Dr. Brigid Hogan, Duke University	N/A
Mouse: ROSA-Tomato (B6.Cg-Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/J)	The Jackson Laboratory	Stock# 007914
Mouse: mTmG (Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/J)	The Jackson Laboratory	Stock# 007576
Software and algorithms
ZEN v2.3	Zeiss Microscopy	https://www.zeiss.com/microscopy/us/products/microscope-software/zen.html
Other
Corn oil	Mazola	N/A
70 μm cell strainer	Corning Falcon	Cat# 352350
20 μm nylon gauze	Fisher Scientific	Cat# 08-801-15
100 mm tissue culture plate	Dot Scientific	Cat# 667621
Phosphate buffered saline	Sigma	Cat# P3813
15 mm round glass coverslip	VWR	Cat# CLS-1760-015
18 mm round glass coverslip	VWR	Cat# 72229-01
6.5 mm Transwell inserts (0.45 μm pore size)	Corning	Cat# 3413
Glass slides	Fisher	12-550-15
24-well cell culture plate	Denville	Cat# T1024
MagRack (magnetic rack)	Sigma	Cat# GE28-9489-64
Cytospin cytocentrifuge	Thermo Fisher Scientific	N/A
MoFlo Astrios cell sorter	Beckman Coulter	N/A