Table 1.
All Quantitative Reverse-Transcription PCR Primers Applied in This Study
| Name | Sequence |
|---|---|
| Human stem cell markers | |
| BMI1 F | GGTACTTCATTGATGCCACAACC |
| BMI1 R | CTGGTCTTGTGAACTTGGACATC |
| LGR5 F | CCTGCTTGACTTTGAGGAAGACC |
| LGR5 R | CCAGCCATCAAGCAGGTGTTCA |
| OCT4 R | CCTGAAGCAGAAGAGGATCACC |
| OCT4 F | AAAGCGGCAGATGGTCGTTTGG |
| CD133 R | CACTACCAAGGACAAGGCGTTC |
| CD133 F | CAACGCCTCTTTGGTCTCCTTG |
| CK7 R | TGTGGATGCTGCCTACATGAGC |
| CK7 F | AGCACCACAGATGTGTCGGAGA |
| HOPX R | ATTCCACCACGCTGTGCCTCAT |
| HOPX F | AGTCTGTGACGGATCTGCACTC |
| SOX2 R | GCTACAGCATGATGCAGGACCA |
| SOX2 F | TCTGCGAGCTGGTCATGGAGTT |
| LRIG1 R | GTGTCATCACCAACCACTTTGGC |
| LRIG1 F | GCAATCTGAGGGTTTGGGTGAC |
| TERT R | GCCGATTGTGAACATGGACTACG |
| TERT F | GCTCGTAGTTGAGCACGCTGAA |
| CD44 R | CCAGAAGGAACAGTGGTTTGGC |
| CD44 F | ACTGTCCTCTGGGCTTGGTGTT |
| NANOG R | CTCCAACATCCTGAACCTCAGC |
| NANOG F | CGTCACACCATTGCTATTCTTCG |
| Mouse stem cell markers | |
| BMI1 F | ACTACACGCTAATGGACATTGCC |
| BMI1 R | CTCTCCAGCATTCGTCAGTCCA |
| LGR5 F | AGAGCCTGATACCATCTGCAAAC |
| LGR5 R | TGAAGGTCGTCCACACTGTTGC |
| OCT4 R | CAGCAGATCACTCACATCGCCA |
| OCT4 F | GCCTCATACTCTTCTCGTTGGG |
| CD133 R | CTGCGATAGCATCAGACCAAGC |
| CD133 F | CTTTTGACGAGGCTCTCCAGATC |
| CK7 R | CGGAGATGAACCGCTCTATCCA |
| CK7 F | CATGAGCATCCTTGATTGCCAGC |
| SOX2 R | AACGGCAGCTACAGCATGATGC |
| SOX2 F | CGAGCTGGTCATGGAGTTGTAC |
| LRIG1 R | TTCAGCCAACGCTACCCTCACA |
| LRIG1 F | TAAGCCAGGTGATGCGTGGTGT |
| TERT R | GAAAGTAGAGGATTGCCACTGGC |
| TERT F | CGTATGTGTCCATCAGCCAGAAC |
| CD44 R | CGGAACCACAGCCTCCTTTCAA |
| CD44 F | TGCCATCCGTTCTGAAACCACG |
| NANOG R | GAACGCCTCATCAATGCCTGCA |
| NANOG F | GAATCAGGGCTGCCTTGAAGAG |
| MEX3A R | AGAGCCTCACGCAACAAGTCTG |
| MEX3A F | CTGGATGCGTTTGATGGTCGCT |
| MUC5AC R | CCACTTTCTCCTTCTCCACACC |
| MUC5AC F | GGTTGTCGATGCAGCCTTGCTT |
| SOX17 R | GCCGATGAACGCCTTTATGGTG |
| SOX17 F | TCTCTGCCAAGGTCAACGCCTT |
| SOX9 R | CACACGTCAAGCGACCCATGAA |
| SOX9 F | TCTTCTCGCTCTCGTTCAGCAG |
| MUC1 R | AGTGCCTCTGACGTGAAGTCAC |
| MUC1 F | GGGAGGGAACTGCATCTCATTC |
| OLFM4 R | GCCTCCAAAAGTGACCTTGTGC |
| OLFM4 F | TGCGTGTGCTGGTGGAAAAGAG |
| HOPX F | GGGTGCTTGTTGACCTTGTT |
| HOPX R | TCTCCATCCTTAGTCAGACGC |
| MGAPDH F | CATCACTGCCACCCAGAAGACTG |
| MGAPDH R | ATGCCAGTGAGCTTCCCGTTCAG |
| HGAPDH F | GTCTCCTCTGACTTCAACAGCG |
| HGAPDH R | ACCACCCTGTTGCTGTAGCCAA |
| Human CAF marker | |
| FAP R | GGAAGTGCCTGTTCCAGCAATG |
| FAP F | TGTCTGCCAGTCTTCCCTGAAG |
| α-SMA R | CTATGCCTCTGGACGCACAACT |
| α-SMA F | CAGATCCAGACGCATGATGGCA |
| VIMENTIN R | AGGCAAAGCAGGAGTCCACTGA |
| VIMENTIN F | ATCTGGCGTTCCAGGGACTCAT |
| FSP1 R | CAGAACTAAAGGAGCTGCTGACC |
| FSP1 F | CTTGGAAGTCCACCTCGTTGTC |
| PDGFRA R | GACTTTCGCCAAAGTGGAGGAG |
| PDGFRA F | AGCCACCGTGAGTTCAGAACGC |
| PDGFRB R | TGCAGACATCGAGTCCTCCAAC |
| PDGFRB F | GCTTAGCACTGGAGACTCGTTG |
| CD29 R | GGATTCTCCAGAAGGTGGTTTCG |
| CD29 F | TGCCACCAAGTTTCCCATCTCC |
| CAV1 R | CCAAGGAGATCGACCTGGTCAA |
| CAV1 F | GCCGTCAAAACTGTGTGTCCCT |
| DESMIN R | CTGAGCAAAGGGGTTCTGAG |
| DESMIN F | ACTTCATGCTGCTGCTGTGT |
| GREMLIN1 R | TCATCAACCGCTTCTGTTACGGC |
| GREMLIN1 F | CAGAAGGAGCAGGACTGAAAGG |
| COL1A1 R | GATTCCCTGGACCTAAAGGTGC |
| COL1A1 F | AGCCTCTCCATCTTTGCCAGCA |
| PERIOSTIN R | TGCCCAGCAGTTTTGCCCAT |
| PERIOSTIN F | CGTTGCTCTCCAAACCTCTA |
| Mouse CAF marker | |
| GREMLIN1 R | AGGTGCTTGAGTCCAGCCAAGA |
| GREMLIN1 F | TCCTCGTGGATGGTCTGCTTCA |
| COL1A1 R | CCTCAGGGTATTGCTGGACAAC |
| COL1A1 F | CAGAAGGACCTTGTTTGCCAGG |
| PERIOSTIN R | CAGCAAACCACTTTCACCGACC |
| PERIOSTIN F | AGAAGGCGTTGGTCCATGCTCA |
| VIM F | CGGAAAGTGGAATCCTTGCAGG |
| VIM R | AGCAGTGAGGTCAGGCTTGGAA |
| FSP1 F | AGCTCAAGGAGCTACTGACCAG |
| FSP1 R | GCTGTCCAAGTTGCTCATCACC |
| CD29 F | CTCCAGAAGGTGGCTTTGATGC |
| CD29 R | GTGAAACCCAGCATCCGTGGAA |
| CAV1 F | CACACCAAGGAGATTGACCTGG |
| CAV1 R | CCTTCCAGATGCCGTCGAAACT |
| Desmin F | GCGGCTAAGAACATCTCTGAGG |
| Desmin R | ATCTCGCAGGTGTAGGACTGGA |
| FAP F | CACCTGATCGGCAATTTGTG |
| FAP R | CCCATTCTGAAGGTCGTAGATGT |
| α-SMA F | CCAGAGCAAGAGAGGGATCCT |
| α-SMA R | TGTCGTCCCAGTTGGTGATG |
| PDGFRA F | GCAGTTGCCTTACGACTCCAGA |
| PDGFRA R | GGTTTGAGCATCTTCACAGCCAC |
| PDGFRB F | GTGGTCCTTACCGTCATCTCTC |
| PDGFRB R | GTGGAGTCGTAAGGCAACTGCA |
BMI, body mass index; CAV1, caveonin 1; CD, cluster of differentiation; CK7, cytokeratin 7; COL1A1, collagen type I a 1; F, forward; FSP1, fibroblast-specific protein 1; HGAPDH, human glyceraldehyde 3-phosphate dehydrogenase; HOPX, Homeodomain-only protein; LGR, Leucine-rich repeat-containing G-protein coupled receptor; LRIG1, Leucine-rich repeats and immunoglobulin-like domains protein 1; MEX3A, Mex-3 RNA Binding Family Member A; MGAPDH, mouse Glyceraldehyde 3-phosphate dehydrogenase; MUC, mucin; NANOG, Tir Na Nog; OCT4, octamer-binding transcription factor 4; OLFM4, Olfactomedin 4; PDGFRB, platelet-derived growth factor receptor b; R, reverse; SOX, SRY-Box Transcription Factor; TERT, Telomerase reverse transcriptase; VIM, vimentin.