Table 3. Histological and molecular data of patients with PXAs.

Case	Central review	Pleomorphism	Mitotic activity	Vascular proliferation	Necrosis	GFAP	S-100	Nestin	Synapto	NFP	NeuN	CD34	IDH1 R132H	Ki67 index (%)	BRAF status*	TERTp status*
1	PXA	++	–	–	–	p	p	p	n	n	n	n	n	0.2
2	PXA	++	–	–	–	fp	p	p	fp	fp	n	n	n	0.9	wt	wt
3	PXA	++	–	–	–	p	p	p	p	fp	n	fp	n	1.8	V600E	wt
4	PXA	+	–	–	–	p	p	p	p	fp	n	n	n	3.5	599insT	wt
5	PXA	++	–	+	–	p	p	p	n	n	n	n	n	3.7
6	PXA	++	–	–	–	fp	p	p	n	n	n	fp	n	4.4
7	PXA	++	–	–	–	p	p	p	fp	p	n	fp	n	0.8
8	PXA	++	–	–	–	fp	p	p	fp	fp	n	p	n	1.9	V600E	wt
9	PXA	++	–	–	–	p	p	p	fp	n	n	n	n	1.2	wt	wt
10	PXA	+	–	–	–	p	p	p	p	n	n	fp	n	5.2	V600E	wt
11	PXA	++	–	–	–	p	p	p	n	n	n	fp	n	0.9	wt	wt
12	PXA	++	–	–	–	p	p	p	p	n	n	fp	n	2.6		wt
13	PXA	+	+	–	–	p	p	p	fp	fp	n	n	n	2.9	V600E	wt
14	PXA	++	–	–	–	p	p	p	n	fp	n	n	n	1.7	599insT	wt
15	PXA	++	–	–	–	p	p	p	fp	p	n	n	n	4.3	wt	wt
16	PXA	++	–	–	–	p	p	p	NA	n	n	p	n	4	V600E	wt
17	PXA	–	–	–	–	p	p	p	n	NA	n	fp	n	0.3	V600E	wt
18	A-PXA	++	+	+	++	p	fp	p	fp	fp	n	n	n	47.5	wt	wt
19	A-PXA	++	+	+	+	p	p	p	n	n	n	n	n	16.7	wt	C228T

*The results were not obtained for BRAF from five samples and for TERTp from four samples because of degradation of the genome structure.

-: none, +: moderate, ++: marked, fp: focally positive, n: negative, NA: not available, p: positive, PXAs: pleomorphic xanthoastrocytoma, synapto: synaptophysin, wt: wild-type.