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. 2020 Dec 29;33(13):108533. doi: 10.1016/j.celrep.2020.108533

Figure 3.

Figure 3

Reduced HSC Compartment in Usp15 Knockout (KO) Mice

Flow cytometry analysis of the hematopoietic primitive populations in 8- to 12-week-old Usp15+/+ and Usp15−/− mice.

(A) Representative FACS profiles of the Lin−, c-Kit+, LSK, and HSC populations. Frequency of HSCs in the live cell population is presented.

(B) Lin−, c-Kit+ cell numbers per million live BM cells.

(C) LSK cell numbers per million live BM cells.

(D) HSC (LSK, CD150+, CD48−/−) cell numbers per million live BM cells.

(E) Frequency of Lin−, c-Kit+, LSK and HSC in BM of Usp15−/− mice was calculated and normalized to Usp15+/+ animals.

Results are from three (Lin−, c-Kit+, and LSK; Usp15+/+ n = 9; Usp15−/−, n = 5) or four (HSC; Usp15+/+, n = 13; Usp15−/−, n = 7) independent experiments. ∗p ≤ 0.05, ∗∗∗∗p ≤ 0.001; n.s., not significant. Error bars represent ± SEM. See also Figure S4.