Table 2.

Details of primers used for qPCR-based mRNA expression analysis

Gene	Primer sequencesa	Amplicon size(bp)
GAPDH	FP 5′ GCCACATCGCTCAGACACCAT 3′	72
	RP 5′ ACCAGGCGCCCAATACG 3′
MN1	FP 5′ CAGAACCCCAACAGCAAAGAA 3′	90
	RP 5′ GACAGACAGGCACTGCAAGTG 3′
IGFBP5	FP 5′ CTACAAGAGAAAGCAGTGCAAACC 3′	62
	RP 5′ TCCACGCACCAGCAGATG 3′
IGF1	FP 5′ GTGCTGCTTTTGTGATTTCTTGA 3′	76
	RP 5′ GCACAGCGCCAGGTAGAAG 3′

^aForward and reverse primers are located in different exons, thus eliminating chance amplification from potential contaminating genomic DNA. FP and RP represent forward and reverse primers, respectively.