Fig. 2.

Blockade of ET_A receptor abolished the natriuretic response to renal medullary GPER1 activation in female ET_B def rats. Urinary Na⁺ excretion (U_NaV) (A), urine flow (UV) (B), and urinary K⁺ excretion (U_KV) (C) were measured during two 20-minute urine collection periods in anesthetized female ET_B def rats or TG control rats: at baseline during the intramedullary infusion of vehicle and 20 minutes after the infusion of the GPER1 agonist G1 (5 pmol/kg per minute) was initiated. ET_A receptor blockade was achieved by intravenous bolus injections of ABT-627 (5 mg/kg) 30 minutes before the beginning of the baseline urine collection period; TG and ET_B def controls received intravenous bolus injections of vehicle. Each line shows data for an individual animal. Statistical comparisons were performed by repeated-measures two-way ANOVA followed by Dunnett’s post hoc tests.