Figure - PMC

Skip to main content

View full-text article in PMC

. Author manuscript; available in PMC: 2021 Jan 7.

Published in final edited form as: J Recept Signal Transduct Res. 2016 Jun 8;37(2):124–132. doi: 10.1080/10799893.2016.1193522

Figure 3. — Metabolic rates are measured by indirect calorimetry in mice using an 8-chamber open-circuit Oxymax system. Briefly, mice are acclimated to the experimental room for 1 week prior and individually housed in acrylic calorimeter chambers through which air with a known O₂ concentration is passed at a constant flow rate. The system automatically withdraws gas samples from each chamber hourly for 24 h and calculates the volumes of O₂ consumed (VO₂) and CO₂ generated (VCO₂). The respiratory quotient (RQ), which is the ratio of VCO₂ to VO₂, is calculated. A. RQ profile for CAM α_1A-AR (squares), CAM α_1B-AR (triangles) mice and WT control (circles). F(2,75)=36.76, p≤ 0.001. Both CAM statistically significant (P<0.001) from WT control using Repeated Measures ANOVA. B. RQ profile for α_1A-AR KO (squares), α_1B-AR KO (triangles), and WT controls (circles). N=6 or 8 mice. (F(2,130)=48.65, p<.001), Both KO statistically significant (p<0.001) from WT control using Repeated measures ANOVA.

Figure 3. — Metabolic rates are measured by indirect calorimetry in mice using an 8-chamber open-circuit Oxymax system. Briefly, mice are acclimated to the experimental room for 1 week prior and individually housed in acrylic calorimeter chambers through which air with a known O₂ concentration is passed at a constant flow rate. The system automatically withdraws gas samples from each chamber hourly for 24 h and calculates the volumes of O₂ consumed (VO₂) and CO₂ generated (VCO₂). The respiratory quotient (RQ), which is the ratio of VCO₂ to VO₂, is calculated. A. RQ profile for CAM α_1A-AR (squares), CAM α_1B-AR (triangles) mice and WT control (circles). F(2,75)=36.76, p≤ 0.001. Both CAM statistically significant (P<0.001) from WT control using Repeated Measures ANOVA. B. RQ profile for α_1A-AR KO (squares), α_1B-AR KO (triangles), and WT controls (circles). N=6 or 8 mice. (F(2,130)=48.65, p<.001), Both KO statistically significant (p<0.001) from WT control using Repeated measures ANOVA.