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. Author manuscript; available in PMC: 2021 Jan 7.

Published in final edited form as: J Recept Signal Transduct Res. 2016 Jun 8;37(2):124–132. doi: 10.1080/10799893.2016.1193522

Figure 5. — Mice were first fasted for 6 hours, anesthetized, then blood collected from the vena cava into 1.5ml polypropylene microfuge tubes. The blood samples were allowed to sit at room temperature for 30 min to coagulate, then centrifuged at 2000x g for 10 min at 4°C. The sera was transferred to fresh tubes and re-centrifuged to remove blood cells. Blood samples were analyzed by the Veterinary Diagnostic Services of Marshfield Laboratories (Cleveland, Ohio) using standard clinical procedures. * p≤ 0.05, statistically significant from WT control. N=6–10 mice.

Figure 5. — Mice were first fasted for 6 hours, anesthetized, then blood collected from the vena cava into 1.5ml polypropylene microfuge tubes. The blood samples were allowed to sit at room temperature for 30 min to coagulate, then centrifuged at 2000x g for 10 min at 4°C. The sera was transferred to fresh tubes and re-centrifuged to remove blood cells. Blood samples were analyzed by the Veterinary Diagnostic Services of Marshfield Laboratories (Cleveland, Ohio) using standard clinical procedures. * p≤ 0.05, statistically significant from WT control. N=6–10 mice.