Figure EV4. The DNA damage‐unresponsive ATM mutant (ATM^2RA) does not affect mitophagy induced by CCCP.

• A
  ATM^2RA U2OS cells were treated for 8 h with 1 μM CCCP and incubated with chloroquine (CLQ) to enhance differences in mitophagy. Anti‐TOM20 (red) was used to visualize mitochondria; anti‐LC3 (green) was used to identify autophagosomes.
• B
  Percentage of mitochondria merging with LC3‐positive puncta calculated by Fiji analysis software using the open‐source plugin ComDet v. 0.3.7. Values are expressed as % of mitochondria (TOM20⁺ particles) co‐localizing with LC3/cell and graphed as boxes (25^th–75^th interquartile range) and whiskers (minimum to maximum showing all points), with central bands representing the median of n ≥ 9 different cells. n.s., not significant.