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. 2020 Nov 23;22(1):e50640. doi: 10.15252/embr.202050640

Figure EV5. Mitochondrial altFUS mutants potentiates TDP‐43 cytoplasmic aggregates.

Figure EV5

  1. Images by confocal microscopy of altFUS (FLAG‐tagged—green) and mitochondria (Tomm20 marker, red) in HeLa cells over‐expressing the empty vector (mock), altFUS‐Flag, altFUS‐P31L‐Flag, altFUS‐A38V‐Flag, altFUS‐A46V‐Flag or altFUS‐R64P‐Flag constructs (representative images from n = 3). The white scale bar corresponds to 10 μm. Deconvolution over a maximum of 30 iterations on the green and red channels was performed for the zoomed in pictures. The white scale bar corresponds to 1 μm.
  2. Images by confocal microscopy of endogenous FUS (red) and altFUS (Flag‐tagged—green) in HeLa cells over‐expressing the empty vector (mock), altFUS‐Flag, altFUS‐P31L‐Flag, altFUS‐A38V‐Flag, altFUS‐A46V‐Flag or altFUS‐R64P‐Flag constructs (representative images from n = 3). The white scale bar corresponds to 10 μm.