Figure 2. LLO^T binds to host cells.

Control HeLa cells (A), and HeLa cells pre-treated with 5 mM methly-β-cyclodextrin (mβCD) (B), were incubated 10 min at 4°C with LLO or LLO^T. (C) HeLa cells pre-treated, or not, with 5 mM mβCD were incubated 10 min at 4°C with LLO D1–3. (D) THP-1 cells were incubated 10 min at 4°C with LLO or LLO^T. (A, B, C, D) After incubation at 4°C, cells were washed and lysed at 4°C, and cell lysates were analyzed by immunoblotting using anti-LLO and anti-actin primary antibodies. Representative immunoblots were selected from at least 3 independent experiments.