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. Author manuscript; available in PMC: 2021 Aug 10.
Published in final edited form as: Vaccine. 2020 Jul 17;38(36):5803–5813. doi: 10.1016/j.vaccine.2020.06.049

Figure 4. Immunization with LLOT plus cholera toxin protects mice against infection by L. monocytogenes.

Figure 4.

Mice were immunized at weekly intervals, for 3 consecutive weeks, by intraperitoneal injection of PBS (negative control), cholera toxin adjuvant (CT, 1 μg), LLOT (20 μg), or LLOT (20 μg) plus cholera toxin (1 μg) (LLOT + CT). On day 28, mice were intravenously inoculated with 2 × 104 L. monocytogenes and sacrificed after 72 h to collect organs and enumerate bacterial colony forming units (CFUs) in the liver (A) and spleen (B). Results are expressed as CFUs/organ and medians are presented. Data are from 3 independent experiments with 5 females per condition in experiment 1, 5 females per condition in experiment 2, and 4 males and 4 females per condition in experiment 3. Statistical significance was calculated using a two-sided Mann-Whitney test, ** P < 0.01.