Figure 7. Immunization with LLO^T plus cholera toxin generates LLO-neutralizing antibodies.

IgGs (15 μg/ml) were purified from pooled sera isolated from 8 mice per immunized group: PBS, LLO^T + CT adjuvant, or LLO^T + Alum adjuvant, and tested for their ability to neutralize 5 nM LLO mixed with human erythrocytes. Erythrocyte lysis of was measured at OD₇₀₀ at 60 sec intervals for 30 min. As hemolysis negative and positive controls, erythrocytes were incubated with PBS or Triton X-100, respectively. Data are representative of 4 independent experiments.