Extended Data Figure 7. AIM2 contributes to CNS cell death during neurodevelopment and in response to ionizing radiation.

(a) Representative 20X images from p5 WT, Ice^−/−, Aim2^−/−, and Gsdmd^−/− mice showing TUNEL⁺ cells (green) in the cerebellum. Images are representative from 2 independent experiments with similar results. (b) Representative images of additional markers of cell death (propidium iodide (PI), grey) in p5 WT and Aim2^−/− mice. (c) Quantification of PI⁺ cells in the cerebellum of p5 WT n=4 and Aim2^−/− n=4 mice; from 1 independent experiment. (d-e) p5 WT and Aim2^−/− mice received either control treatment or 14 Grays (14 Gy) of ionizing radiation (IR) to induce DNA damage. Brains were harvested 6 hrs later and then TUNEL assay staining was conducted on cerebellar sections to evaluate cell death. (d) Representative 20X images showing TUNEL staining in the cerebellum of untreated and irradiated p5 WT and Aim2^−/− mice; from 3 independent experiments with similar results. (e) Quantification of number of TUNEL⁺ cells in the cerebellums of untreated and irradiated WT (n=11 untreated; n=9 IR) and Aim2^−/− (n=9 untreated; n=8 IR) mice; from 3 independent experiments. All n values refer to the number of mice used. Error bars depict mean ± s.e.m. Statistics calculated by unpaired two-tailed Student’s t-test.