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. 2021 Jan 7;58(5):2145–2157. doi: 10.1007/s12035-020-02250-4

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — Developmental time course of GFRAL in the human hippocampus. a Representative blot of relative GFRAL levels in the hippocampus in infants (n = 8/group), toddlers (n = 8/group), preschoolers (n = 7/group), adolescents (n = 8/group), and adults (n = 8/group). b Membrane stain shows total protein loading across subjects. c Normalized densitometry values were analyzed by Kruskal-Wallis 1-way ANOVA. * indicates post hoc significance vs. infants. # indicates post hoc significance vs. toddlers. Data were significant at p < 0.05. Box plots show minimum, maximum, IQR, and median. The asterisk above GFRAL denotes that the band analyzed by densitometry matches the predicted kDa