Table 1.
Primary antibody reagent details. High-specificity monoclonal antibodies were used for the detection of CSH receptor components and MBP. In addition, a higher sensitivity polyclonal antibody targeting GFRAL was also employed
| # Target | Vendor | Host | Clonality | Cat # | Lot # |
|---|---|---|---|---|---|
| 1 MBP | Cell Signaling Technology | Rabbit | Monoclonal | 78896 | 2 |
| 2 Integrin aV | Cell Signaling Technology | Rabbit | Monoclonal | 60896 | 1 |
| 3 Integrin β5 | Cell Signaling Technology | Rabbit | Monoclonal | 3629 | 1 |
| 4 GFRAL | R&D System | Rabbit | Monoclonal | MAB9697 | CMAT 0219111 |
| 5 GFRAL | Cusabio | Rabbit | Polyclonal | CSB-PA751020 LA01HU | F0927A |