Figure 4.

PT‐BsAb redirects HSCs to the injured heart. Flow cytometry quantification results of platelet‐conjugated HSCs (CD34⁺CD42b⁺) in a) the lungs and b) the heart. c) Flow cytometry quantification results of PT‐BsAbs binding of HSCs‐platelets (CD34⁺CD42b⁺DyLight 633⁺). HSCs and platelets were separately stained with CD34 antibody and CD42b antibody, while PT‐BsAbs were pre‐labeled with DyLight 633. d) Representative confocal images showing the accumulation of HSCs after the inhalation of PT‐BsAbs or NT‐BsAbs, i.v. injection of PT‐BsAbs in the border zone of MI mice, and inhalation of PT‐BsAbs in sham mice at 6 h. Cardiomyocytes were stained with α‐SA (red), while HSCs were stained with CD34 antibody (green). Nuclei were stained with DAPI (blue). Scale bar, 10 µm. e) Quantification of HSC accumulation in the heart 6 h after various administrations. f,g) Western blot analysis of CD34 expression in the heart 6 h after various treatments. N = 3.