Table 3.

Chimerism results of the different isolation methods using NGS and ddPCR techniques

Marker ID	Chromosome	Recipient	Donor	Control gDNA		MagNAPure		IDEAL		LabTurbo24 AB		Chemagic 360
				100%	15%	10%	1%	10%	1%	10%	1%	10%	1%
NGS chimerism
1	1	+/−	−/−	100	14.7	16.2**	0.1*	0**	0*	21.8*	4.6	10.6**	5.7*
3	1	+/−	+/+	100	12.8*	3.6**	0*	0**	8.8*	12.7*	2.6	0**	3.3**
8	6	+/−	−/−	100*	18.8*	13.9*	0	0**	2.3*	16.2*	4.9*	12.8**	4*
9	7	+/−	+/+	100*	30.2*	35.8**	0*	0**	2.9*	12.3*	1.9	0**	0.7**
11	8	−/−	+/+	99.1*	18.8*	19.4*	0*	0**	1.5*	10.4*	2.3	5.7**	1.2*
12	9	+/+	−/−	98.1	16.5*	10.6**	0*	0**	3.9*	12.7*	1.2	15**	2.4*
22	20	−/−	+/+	98.9	17.1	7.1**	0**	100**	2.6**	24*	1.8	0**	1.4**
23	22	+/−	+/+	100*	16*	14.8*	0	0**	0*	17.5*	2.8*	8.1**	1*
Average (%)				99.5	18.1	15.2	0	12.5	2.7	16	2.7	6.5	2.4
SD				0.7	5.3	9.8	0	35.4	2.8	4.9	1.3	6.1	1.8
ddPCR chimerism
Total droplets				22,512	21,496	22,286	22,685	21,481	21,253	16,812	18,665	19,207	19,115
RPP30 positive droplets				13,184	11,256	94	84	40	29	124	128	55	40
Y-marker positive droplets				7121	914	0	0	0	0	4	1	0	0
% chimerism (Y-marker heterozygous × 2)				108%	16%	NC	NC	NC	NC	6%	< DL NC	NC	NC

Quality warning: * (yellow): coverage: 1000–10,000 X, ** (red): coverage < 1000 X

NC not calculated, DL detection limit