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. 2021 Jan 6;18:1. doi: 10.1186/s12989-020-00396-6

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 7 — Ultrastructural detection of Co₃O₄-NPs in rat ventricular cardiomyocytes. Panels a and b: Transmission Electron Microscopic (TEM) images of a CTRL cardiomyocyte illustrating the regular distribution of mitochondria (Mit) and myofibrils (MF) and the scattered accumulation of small electrondense glycogen granules (*). Panels c-f: Co₃O₄-NPs (arrows) were detected within the mitochondria (Mit; c-d) and at the interface between mitochondria and myofibrils (MF; e-f) in cardiomyocytes exposed to 5 μg/ml (NPc₅) and 50 μg/ml (NPc₅₀) Co₃O₄-NPs, respectively. N: cardiomyocyte nucleus. Scale bars: 2 μm in a, c, d; 0.5 μm in b, d, f.