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Newly designed primers to amplify the whole genome sequence of DCH
| Primer | Sequence | Base pair | Annealing Temperature |
|---|---|---|---|
| DCH A | F: 5’-TGGGCAACATTACCTCAGGTCC-3’ | 1700 | 56℃ |
| R: 5’-GGAACAAAAGAGAACGCACAGG-3’ | |||
| DCH B | F: 5’-GGTCTGACGCCCAGGTTATG-3’ | 1700 | 56℃ |
| R: 5’-ACCACGAGTCTGCACTCTGC-3’ | |||
| DCH C | F: 5’-CTCAGGTCTTTGCCCACTCA-3’ | 1155 | 55℃ |
| R: 5’-AGCTGACTCCTCCCAACAGT-3’ | |||
| DCH D | F: 5’-AACTAAGCATGAACTCCGCC-3’ | 1173 | 55℃ |
| R: 5’-TGGGCCAACAGGTGCAATTT-3’ |
