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. 2021 Jan 6;14:6. doi: 10.1186/s13045-020-01021-x

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — HOMER3 interacts with tyrosine-protein kinase c-Src. a Tyr phosphorylation levels of β-Catenin were examined in control and HOMER3-overexpressing MDA-MB-231 and SUM159PT cells with or without c-Src knockdown. b Schematic illustration of two conserved PxxF (x, any amino acid) motifs in c-Src protein that might be recognized by the EVH1 domain of HOMER3. c Immunoprecipitation (IP) assays determined the interaction between endogenous HOMER3 and c-Src in MDA-MB-231 cells. d IP assays were performed in 293 T cells transfected with HA-HOMER3 and MYC-c-Src constructs. e Left panel: Schematic illustration of HOMER3 truncated constructs. Right panel: The 293 T cells were transfected with indicated HA-tagged HOMER3 truncations and MYC-c-Src, followed by IP assays with HA-beads to examine their interaction with c-Src. f Left panel: Sketch of MYC-c-Src truncations. Right panel: The 293 T cells were transfected with indicated MYC-c-Src truncations and HA-HOMER3, followed by IP assays with MYC-beads to examine their interaction with HOMER3. g IP assays examined the interactions between HOMER3 and wild-type (wt) or mutant (P61L and P307L) c-Src proteins. h MDA-MB-231 cells were first stimulated with EGF for a short time, and then fluorescence immunostaining of HOMER3 and c-Src was performed to examine their colonization. Statistical analysis indicated the percentage of cells that showed membrane colonization of c-Src and HOMER3. ***P < 0.001