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. 2021 Jan 6;14:3. doi: 10.1186/s13072-020-00376-2

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Functional analysis of H1T2 bound promoter-TSS regions in rat spermatids genome. a Gene ontology analysis of the 321 genes whose promoter-TSS regions are bound to H1T2. David software was used for the analysis and the results are shown in the figure. GO biological processes are represented in the figure with P values. X-axis represents the number of genes associated to the GO term. b Distribution of H1T2 peaks from ± 3 kb centered around TSS of genes in the spermatids genome and heatmap showing the same observation ngs. Plot showing distribution of H1T2 peaks throughout the gene. c GO analysis of 321 genes according to molecular function represented as pie diagram. The number of genes associated to the GO term is given in the pie chart. 23 transcription factors whose promoter-TSS is bound to H1T2 are highlighted in the pie diagram. d Integrative Genomics Viewer (IGV) outputs of representative genomic regions showing the occupancy of H1T2 at various transcription factors of the spermatid genome

Fig. 4 — Functional analysis of H1T2 bound promoter-TSS regions in rat spermatids genome. a Gene ontology analysis of the 321 genes whose promoter-TSS regions are bound to H1T2. David software was used for the analysis and the results are shown in the figure. GO biological processes are represented in the figure with P values. X-axis represents the number of genes associated to the GO term. b Distribution of H1T2 peaks from ± 3 kb centered around TSS of genes in the spermatids genome and heatmap showing the same observation ngs. Plot showing distribution of H1T2 peaks throughout the gene. c GO analysis of 321 genes according to molecular function represented as pie diagram. The number of genes associated to the GO term is given in the pie chart. 23 transcription factors whose promoter-TSS is bound to H1T2 are highlighted in the pie diagram. d Integrative Genomics Viewer (IGV) outputs of representative genomic regions showing the occupancy of H1T2 at various transcription factors of the spermatid genome