Fig. 3.

MiR-203 is identified as a direct target of circ_0000396. a The putative binding sits between circ_0000396 and miR-203 were predicted. b–d The interaction between circ_0000396 and miR-203 was confirmed by the dual-luciferase reporter assay (Mann–Whitney U-test) and pull-down assay in RASFs (ANOVA). e, f Expression of miR-203 in RA synovial tissues and healthy control tissues as well as in RASFs and NSFs was detected by qRT-PCR (Student’s t test). g qRT-PCR analysis of miR-203 expression in RASFs transfected with pcNDA, circ_0000396, si-NC, or si-circ_0000396 was conducted (Student’s t test). ^*p < 0.05, ^**p < 0.01, ^***p < 0.001, ^****p < 0.0001