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. 2021 Jan 6;21:13. doi: 10.1186/s12935-020-01707-9

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 8 — PPP2R2B downregulation promoted M1 phenotype polarization and migration capability. a Immunofluorescence assay. b Q-PCR assay validated whether the THP-1 monocytes had been successfully polarized into M1 or M2 macrophages. c Q-PCR assay identified that PPP2R2B was significantly upregulated in M1 macrophages compared with M2 macrophages. d Western blotting evaluated the efficacy of PPP2R2B expression. e The schematic diagram of co-culture system. f Cocultured macrophages exhibited different PPP2R2B expression. g Altered expression of polarization-specific genes in macrophages cocultured with TNBC cells transfected with empty or PPP2R2B overexpressing vector, respectively. upper panel: Cocultured with MDA-MB-231 cells; lower panel: Cocultured with MDA-MB-468 cells. h, i Cocultured macrophage was selected to perform the transwell assay. j, k Transwell assay identified that PPP2R2B-educated macrophages could significantly suppress TNBC cells migration. ns (Not significant), *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001