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. 2021 Jan 6;25:9. doi: 10.1186/s13054-020-03444-x

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — VA-ECMO induced increased in immature circulating neutrophils. a Total neutrophils were determined by blood count formula at admission or VA-ECMO initiation (D0), 24 h and 4 days (D1 and D4) (n = 9 Control (Ctrl) group, n = 9 VA-ECMO group). Mature and immature peripheral blood neutrophil counts were determined by flow cytometry at admission or VA-ECMO initiation (D0), 24 h and 4 days (D1 and D4) (n = 8 Control (Ctrl) group, n = 9 VA-ECMO group). b Surface marker of neutrophil degranulation (CD35, CD63, CD66b) was compared by flow cytometry at admission or VA-ECMO initiation (D0), 24 h and 4 days (D1 and D4) (n = 8 Ctrl group, n = 9 VA-ECMO group). Data presented as ratio of mean fluorescence intensity ratio (rrMFI), defined as the ratio of rMFI between D0 and D1 or D4. C. Expression of C5a receptor on mature and immature neutrophils compared by flow cytometry at admission or VA-ECMO initiation (D0), 24 h and 4 days (D1 and D4) (n = 8 Ctrl group, n = 9 VA-ECMO group). Data expressed as rMFI