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. 2021 Jan 6;14:19. doi: 10.1186/s13071-020-04506-3

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — Absence of L. loa infection in nulliparous Chrysops and Mansonella perstans samples. a Nulliparous samples, b M. perstans (M.P.) samples compared to NTC and positive control (PC). Before amplification (T₀), reaction solutions are pink. Samples remained pink after the assay, indicating negativity, as shown on the photographs after a 40-min amplification (T₄₀). Nulliparous Chrysops are flies that have never taken a blood meal; as such, samples should remain negative in the assay