Fig. 1.

General proteolytic potential of vAh extracellular proteins (ECPs) secreted under biofilm and planktonic growth. The general proteolytic potential of biofilm and planktonic secretomes was measure using HiLyteFluor 488-labeled casein as a substrate. Secreted protein from each condition was incubated at 30 °C with labeled casein and fluorescent intensity was measured at Ex/Em = 490 nm/520 nm every five minutes for one hour. Data were plotted as relative fluorescence units versus time for each sample. Trypsin served as a positive control, and sterile, deionized water served as a negative control. Three individual experiments were performed, and all samples were performed in triplicate. Statistical analysis consisted of one-way ANOVA followed by Tukey’s multiple comparisons post-test with significance set at p < 0.05