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. 2020 Dec 22;23(2):160. doi: 10.3892/mmr.2020.11799

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Copyright: © Park et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 2. — Effect of age on the paracrine potential of ADSCs. ADSCs were cultured and the culture medium was collected for the quantification of cytokines. Quantification for (A) VEGF, (B) HGF, (C) SDF-1α and (D) TGF-β was performed using ELISA. (E) Experimental scheme for Matrigel assay with EPC. Conditioned medium of ADSC was treated with EPCs on Matrigel. (F) Representative images for tube formation. Data are presented as the mean ± SD of at least three replicates for each group. Scale bar, 100 µm. **P<0.01 and ***P<0.001. ADSCs, adipose-derived stem cells; HGF, hepatocyte growth factor; SDF-1α, stromal cell-derived factor-1α; TGF-β, transforming growth factor-β; VEGF, vascular endothelial growth factor; EPC, endothelial progenitor cell.