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. 2020 Dec 22;23(2):160. doi: 10.3892/mmr.2020.11799

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Copyright: © Park et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 3. — Effect of age on the osteogenic differentiation potential of ADSCs. (A) Osteogenic induction was performed for 20 days. ADSCs from the young or elderly were seeded, and the medium was replaced with fresh medium every 3 days. ADSCs were fixed after osteogenic induction and stained using Alizarin red S. Calcium deposition was observed as red color of Alizarin red S solution. Scale bar, 100 µm. (B) Alizarin red was quantified. (C) Measurement of BMP-2 in conditioned medium was carried out using an ELISA. (D) Expression levels of BMPR1A in ADSCs were determined by western blotting. Data are presented as the mean ± SD of at least three replicate wells for each group. **P<0.01 and ***P<0.001. ADSCs, adipose-derived stem cells; BMP-2, bone morphogenetic protein 2; NS, not significant; BMPR1A, bone morphogenetic protein receptor 1A.