Figure 4.
Effect of age on the adipogenic differentiation potential of ADSCs. (A) Adipogenic induction of ADSCs was performed over a period of 15 days. ADSCs were seeded at a density of 5×105 cells/well, and the medium was replaced with a fresh medium every 3 days. Following induction, cells were fixed and stained using Oil Red O. Scale bar, 100 µm. (B) Quantification of adipocytes stained with Oil Red O. Data are presented as the mean ± SD of at least three replicates for each group. ***P<0.001. ADSCs, adipose-derived stem cells.