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. 2020 Dec 22;23(2):165. doi: 10.3892/mmr.2020.11804

Figure 5.

Figure 5.

Effects of miR-126 on the levels of inflammatory-related cytokines in the presence of SIRT1-siRNA or Con-siRNA in OGD/R-exposed HUVECs. HUVECs were pre-transfected with SIRT1-siRNA or Con-siRNA for 30 min and then transfected with miR-126 m or miR-NC prior to OGD (4 h)/R (24 h) exposure. The levels of (A) TNF-α, (B) IL-1β, (C) IL-6 and (D) IL-10 were measured using ELISA kits and the concentrations (µg/mg protein) were calculated using a standard calibration curve. Data are presented as the mean ± SD of at least three independent experiments. *P<0.05 and **P<0.01 vs. control group; #P<0.05 and ##P<0.01 vs. miR-NC + OGD/R group; &P<0.05 and &&P<0.01 vs. miR-126 m + OGD/R + Con-siRNA group. miR-126 m, miR-126 mimics; miR-NC, miR-negative control; miR, microRNA; OGD, oxygen-glucose deprivation; R, reoxygenation; HUVEC, human umbilical vein endothelial cell; SIRT1, NAD-dependent protein deacetylase sirtuin-1; siRNA, small interfering RNA; Con-siRNA, negative control siRNA; TNF-α, tumor necrosis factor-α; IL, interleukin.