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. Author manuscript; available in PMC: 2021 Mar 1.
Published in final edited form as: Nat Med. 2020 Jul 13;26(9):1452–1458. doi: 10.1038/s41591-020-0964-7

Extended Data Fig. 7. Monocytic myeloid-derived suppressor cells isolated from tumors of mice exposed to MI or sham surgery do not alter the proliferation of T cells in vitro.

Extended Data Fig. 7

(a) Representative flow plots of CD11b-Ly6C-CD8+ T cells from MDSC:T cell suppression assay, representing % IFNγ +, TNFα+, Granzyme B (GrB)+ populations. (b,c) Purified splenic CD8+ T cells from naïve mice were stimulated with αCD3/αCD28 for 72 hours in the presence of mMDSCs isolated from tumors from mice exposed to MI (n=5) or sham surgery (n=7), and CD8+ T cell proliferation was assessed by % Ki67 (b) and replication and division index (c) using a cell trace proliferation dye. (a,b,c) two independent experiments were conducted. Data are the mean ± s.e.m. P values were calculated using or two-tailed unpaired Student’s t-test (b,c), where relevant comparisons were not significant (p>0.05)