Figure 1.

Effects of palmitate in INS-1 cells injury, autophagy and FoxO1. (A) Cell Counting Kit-8 assay was used to examine the viability of INS-1 cells, which were treated with various concentrations of palmitate (0, 0.1, 0.3 and 0.5 mmol/l). Data were analyzed by unpaired t-test and are presented as the mean ± SD (n=3). *P<0.05 vs. Con group. Western blotting, using GAPDH as a control, was conducted to determine autophagy-related protein (B) LC3 and (C) FoxO1 expression levels. CON, control; FoxO1, Forkhead box O1; LC3, microtubule-associated protein 1 light chain3.