Fig. 2.

Signal intensity of PD-L1 in LuCa tissues after sample deglycosylation. (A) Representative images show the samples stained with each of the 4 PD-L1 mAbs with or without sample deglycosylation. Bar = 200 μm. (B) Heatmap represents the expression levels of PD-L1 after staining with 4 PD-L1 mAbs with or without sample deglycosylation. Red: high quantitative value, blue: low quantitative value. (C) H-score of PD-L1 signal intensity after staining with 4 PD-L1 mAbs with or without sample deglycosylation. (D) Fold change (FC) of the PD-L1 H-score and the corresponding number of cases. (E) Data points represent the mean H-score of each sample as calculated by HALO software. Superimposed points indicate identical values. (F) The domain structure and glycosylation sites of PD-L1