Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Jan 6;11:3. doi: 10.1186/s13578-020-00509-w

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 6 — Knock-down of Stra6 attenuates liver fibrosis in vivo. a Delivery of a FAM-tagged siRNA targeting Stra6 to the normal or TAA-treated mouse liver using a vitamin A-liposome complex. The FAM-tagged siRNA targeting Stra6 is shown in green, STRA6 is shown in red, retinol is shown in blue, and the nuclei are shown in white. VitaminA-liposome complex only-treated group: n = 3; vitaminA-liposome complex and TAA-treated group: n = 3; TAA/siStra6 group: n = 5. The experiments were performed three times independently. Retinol or αSMA-positive area % per 0.06 mm² were quantified using Image J program in five pictures for each group. Scale bar of lower magnification: 25 μm; scale bar of higher magnification: 5 μm. b The effects of siRNA-mediated knock-down of Stra6 on the expression levels of the mRNAs encoding STRA6, FASN, and SREBP1 in normal and TAA-treated mouse liver tissues. VitaminA-liposome complex only-treated group: n = 3; vitaminA-liposome complex and TAA-treated group: n = 3; TAA/siStra6 group: n = 5. The experiments were performed three times independently. P-values were determined using unpaired Student’s t-tests in Prism8. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. c Oil red-O staining showed the accumulation of triglyceride in TAA- and siStra6-treated mouse liver samples. VitaminA-liposome complex only-treated group: n = 3; vitaminA-liposome complex and TAA-treated group: n = 3; TAA/siStra6 group: n = 5. The experiments were performed three times independently. Scale bar of the upper panel: 5 μm; scale bar of the lower panel: 2 μm. d Quantification of the oil red-O staining shown in (c). VitaminA-liposome complex only-treated group: n = 3; vitaminA-liposome complex and TAA-treated group: n = 3; TAA/siStra6 group: n = 5. Five pictures of each sample were analyzed using ImageJ. P-values were determined using unpaired Student’s t-tests in Prism8. ****P < 0.0001. e MT staining of the extracellular matrix and picro-sirius staining of collagen in the VitaminA-liposome complex only-, vitaminA-liposome complex and TAA-treated, and TAA plus siStra6-treated groups. Hematoxylin and eosin (H&E) staining was also performed. Scale bar: 10 μm. f Quantification of the picro-sirius red staining shown in e VitaminA-liposome complex only-treated group: n = 3; vitaminA-liposome complex and TAA-treated group: n = 3; TAA/siStra6 group: n = 5. Four pictures of each sample were analyzed using ImageJ. P-values were calculated using unpaired Student’s t-tests in Prism8. ****P < 0.0001. g, h The levels of aspartate aminotransferase (g) and alanine aminotransferase (h) in mouse serum. Duplicates of each sample were performed. VitaminA-liposome complex only-treated group: n = 3; vitaminA-liposome complex and TAA-treated group: n = 3; TAA/siStra6 group: n = 5. P-values were calculated using unpaired Student’s t-tests in Prism8. **P < 0.01, ****P < 0.0001

Fig. 6 — Knock-down of Stra6 attenuates liver fibrosis in vivo. a Delivery of a FAM-tagged siRNA targeting Stra6 to the normal or TAA-treated mouse liver using a vitamin A-liposome complex. The FAM-tagged siRNA targeting Stra6 is shown in green, STRA6 is shown in red, retinol is shown in blue, and the nuclei are shown in white. VitaminA-liposome complex only-treated group: n = 3; vitaminA-liposome complex and TAA-treated group: n = 3; TAA/siStra6 group: n = 5. The experiments were performed three times independently. Retinol or αSMA-positive area % per 0.06 mm² were quantified using Image J program in five pictures for each group. Scale bar of lower magnification: 25 μm; scale bar of higher magnification: 5 μm. b The effects of siRNA-mediated knock-down of Stra6 on the expression levels of the mRNAs encoding STRA6, FASN, and SREBP1 in normal and TAA-treated mouse liver tissues. VitaminA-liposome complex only-treated group: n = 3; vitaminA-liposome complex and TAA-treated group: n = 3; TAA/siStra6 group: n = 5. The experiments were performed three times independently. P-values were determined using unpaired Student’s t-tests in Prism8. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. c Oil red-O staining showed the accumulation of triglyceride in TAA- and siStra6-treated mouse liver samples. VitaminA-liposome complex only-treated group: n = 3; vitaminA-liposome complex and TAA-treated group: n = 3; TAA/siStra6 group: n = 5. The experiments were performed three times independently. Scale bar of the upper panel: 5 μm; scale bar of the lower panel: 2 μm. d Quantification of the oil red-O staining shown in (c). VitaminA-liposome complex only-treated group: n = 3; vitaminA-liposome complex and TAA-treated group: n = 3; TAA/siStra6 group: n = 5. Five pictures of each sample were analyzed using ImageJ. P-values were determined using unpaired Student’s t-tests in Prism8. ****P < 0.0001. e MT staining of the extracellular matrix and picro-sirius staining of collagen in the VitaminA-liposome complex only-, vitaminA-liposome complex and TAA-treated, and TAA plus siStra6-treated groups. Hematoxylin and eosin (H&E) staining was also performed. Scale bar: 10 μm. f Quantification of the picro-sirius red staining shown in e VitaminA-liposome complex only-treated group: n = 3; vitaminA-liposome complex and TAA-treated group: n = 3; TAA/siStra6 group: n = 5. Four pictures of each sample were analyzed using ImageJ. P-values were calculated using unpaired Student’s t-tests in Prism8. ****P < 0.0001. g, h The levels of aspartate aminotransferase (g) and alanine aminotransferase (h) in mouse serum. Duplicates of each sample were performed. VitaminA-liposome complex only-treated group: n = 3; vitaminA-liposome complex and TAA-treated group: n = 3; TAA/siStra6 group: n = 5. P-values were calculated using unpaired Student’s t-tests in Prism8. **P < 0.01, ****P < 0.0001